MersV1, Main, Exploration, bibRecord, 004770

In vivo primary induction of virus-specific CTL by immunization with 9-mer synthetic peptides

Identifieur interne : 004770 ( Main/Exploration ); précédent : 004769; suivant : 004771

In vivo primary induction of virus-specific CTL by immunization with 9-mer synthetic peptides

Auteurs : Xianzheng Zhou [Suède] ; Louise Berg [Suède] ; Ussama M. Abdel Motal [Suède] ; Mikael Jondal [Suède]

Source :

Journal of Immunological Methods [ 0022-1759 ] ; 1992.

RBID : ISTEX:7C9B485472D1654106BEE83E0785B006A8CBE96E

Descripteurs français

KwdFr :
- Animaux, Antigènes d'histocompatibilité de classe I (immunologie), Cytotoxicité immunologique, Données de séquences moléculaires, Femelle, Immunisation, Lymphocytes T cytotoxiques (immunologie), Peptides (immunologie), Souris, Souris de lignée C57BL, Séquence d'acides aminés, Virus de la grippe A (immunologie).
MESH :
- immunologie : Antigènes d'histocompatibilité de classe I, Lymphocytes T cytotoxiques, Peptides, Virus de la grippe A.
Pascal (Inist)
- Animaux, Cytotoxicité, Cytotoxicité immunologique, Données de séquences moléculaires, Femelle, Immunisation, In vivo, Induction, Influenzavirus A, Lymphocyte T, Peptide, Produit synthétique, Souris, Souris de lignée C57BL, Séquence d'acides aminés.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Cytotoxicity, Cytotoxicity, Immunologic, Female, Histocompatibility Antigens Class I (immunology), Immunization, In vivo, Induction, Influenza A virus (immunology), Influenzavirus A, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Mouse, Peptides, Peptides (immunology), Synthetic product, T-Lymphocyte, T-Lymphocytes, Cytotoxic (immunology).
MESH :
- chemical , immunology : Histocompatibility Antigens Class I, Peptides.
- immunology : Influenza A virus, T-Lymphocytes, Cytotoxic.
- Amino Acid Sequence, Animals, Cytotoxicity, Immunologic, Female, Immunization, Mice, Mice, Inbred C57BL, Molecular Sequence Data.
Teeft :
- Cell response, Complete medium, Cytotoxic, Dynabeads system, Effeclor target ratio, Effector cells, Immune spleen cells, Immunization, Influenza, Injection site, Intravenous injection, Karolinska institute, Lmmunol, Lymphocyte, Lytic activity, Major histocompatibility, Optimal conditions, Optimal length, Peptide, Peptide vaccination, Primary cytotoxic, R6tzschke, Restimulated, Restimulation, Short peptides, Spleen, Spleen cells, Stimulator cells, Synthetic peptides, Target cells, Vaccination, Viral proteins, Virus strains, Vivo.

Abstract

Abstract: A primary cytotoxic T lymphocyte (CTL) response in vivo requires antigen presentation by cytosolic processing and can not in general be obtained by vaccination with soluble proteins. In the present work we have found that vaccination of mice with pre-processed synthetic peptides, corresponding to endogenous 9-mers produced in influenza A virus-infected cells, resulted in strong primary CTL responses. The generated CTL efficiently killed virus-infected target cells with preference for viral strains having the identical amino acid sequences to the peptides used for immunization. The optimal conditions for a primary in vivo CTL response was obtained with 100 μg peptide dissolved in incomplete Freund's adjuvant and injected s.c. at the base of tail. Spleen cells which had been primed 7–10 days earlier were restimulated for 5 days in vitro, using an optimal low peptide concentration (0.05 μM) and tested against virus-infected and peptide-treated target cells. The peptide-induced CTL were major histocompatibility complex class I restricted and CD8 positive.

Url:

https://api.istex.fr/ark:/67375/6H6-RDC9V5JS-Z/fulltext.pdf

DOI: 10.1016/0022-1759(92)90322-K

Affiliations:

Suède

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<term>Female</term>
<term>Histocompatibility Antigens Class I (immunology)</term>
<term>Immunization</term>
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<term>Induction</term>
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<front><div type="abstract" xml:lang="en">Abstract: A primary cytotoxic T lymphocyte (CTL) response in vivo requires antigen presentation by cytosolic processing and can not in general be obtained by vaccination with soluble proteins. In the present work we have found that vaccination of mice with pre-processed synthetic peptides, corresponding to endogenous 9-mers produced in influenza A virus-infected cells, resulted in strong primary CTL responses. The generated CTL efficiently killed virus-infected target cells with preference for viral strains having the identical amino acid sequences to the peptides used for immunization. The optimal conditions for a primary in vivo CTL response was obtained with 100 μg peptide dissolved in incomplete Freund's adjuvant and injected s.c. at the base of tail. Spleen cells which had been primed 7–10 days earlier were restimulated for 5 days in vitro, using an optimal low peptide concentration (0.05 μM) and tested against virus-infected and peptide-treated target cells. The peptide-induced CTL were major histocompatibility complex class I restricted and CD8 positive.</div>
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Serveur d'exploration MERS

In vivo primary induction of virus-specific CTL by immunization with 9-mer synthetic peptides

In vivo primary induction of virus-specific CTL by immunization with 9-mer synthetic peptides

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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